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Abstract

The present study was designed to explore the anti-inflammatory effect and antioxidant potential of the methanolic extract of Marrubium vulgare leaves, a popular traditional medicinal herb. In vivo anti-inflammatory activity was evaluated by the paw edema assay induced by Carrageenin in rat model, while antioxidant activity was evaluated by two tests: Assay of free radical-scavenging activity on DPPH and Ferric reducing antioxidant plasma (FRAP). Total phenolic and total flavonoid contents of the crude methanol extract were also determined by Folin-Ciocalteu's phenol reagent and by aluminium chloride method, respectively.

The results showed that methanol extract had high level of polyphenols (195 ± 0.06 mg GAE/g extract) and flavono'ids (93.12 ± 0.17 mg QE/g extract). With regards to IC50 values (50% inhibitory concentration) of scavenging abilities of the DPPFI radical, methanol extract (MeOFiE) exhibited important antioxidant capacity with IC50 of 12.42 pg/ml and showing also a dose dependent manner ferric reducing capacity with a value equal 50.01i0.24 pg EAA/g of extract compared to ascorbic acid that was used as a witness in the trial. Assessment of anti-inflammatory activity showed that oral administration of MeOFIE at a dose of 200 mg/kg in rats treated with carrageenin causes a significant decrease (87.3 ± 0.25%) of inflammation compared with standard diclofenac (positive control) which showed 85.52 ± 0.47% protection in this test. The analysis of C-reactive protein showed the absence of this protein in the plasma of rats treated with MeOFiE of the plant. Phytochemical screening of MeOFiE allowed the identification of several pharmaceutical drugs such as tannins/polyphenols, flavono'ids, terpenoids, steroids and alkaloids which may responsible for pharmacological properties. In addition, this study allows the identification of new flavonoid: "Sophorin" (C27H30O16) which revealed for the first time by 7T-Chromatography on silica gel GF254

CONCLUSION

In the current study, methanolic extract of M. vulgare leaves has both anti-inflammatory and antioxidant activities which could be due to the richness of the methanol extract in polyphenols and flavonoTds particularly quercetin and sophorin. Many other compounds such as glycosidic phenylpropanoi'd esters, terpenoids and alkaloids were previously identified may be participating in these activities. The result obtained justifies the use of the plant species by traditional medicine practitioners in Algeria. However, more studies are needed to further elucidate the mechanism of the anti-inflammatory and antioxidant actions of Marrubium vulgare. It is important to remember that these results were obtained in rats. It is therefore essential to carry out experiments at first in another animal model, and then in a second time in humans, to obtain confirmation of the potential of this plant.

Source:https://www.researchgate.net/publication/311206266_Evaluation_of_in_vitro_antioxidant_and_in_vivo_anti-inflammatory_potentialof_white_Horehound_Marrubium_vulgare_Leaves